- Automatic calibration Function
- Wavelength range: 190-850nm
- Sample capacity: 0. 5-2μl
- No need for computer connection
Microvolume UV/ VIS (Nano) Spectrophotometer, SP-MUV1000, SP-MUV1000F
Description
Micro Volume Spectrophotometer
It is an instrument used to detect DNA, RNA purity, concentration, and protein concentration. It can quickly measure the concentration of nucleic acid.We can use two detection modes: pedestal and cuvette, Quick, accurate and repeatable micro-measurement can be carried out without dilution.Please point the sample directly on the sample plate. No accessories such as cuvettes or capillaries are required. After the measurement, you can choose to wipe off the sample directly or use a pipette to recover the sample. The steps are simple and fast.
1. 7-inch high-definition capacitive touch screen, no need for computer connection, and it can be detected by a single machine.
2. Detection wavelength range: 190-850nm
3. Only 0.5—2μL sample is required for base measurement, and dilution is not required even for high concentration samples.
4. It has an OD600 optical path detection system and a cuvette mode, which is convenient for the detection of the concentration of bacteria, microorganisms and other culture solutions.
5. The closing of the detection arm can be detected automatically.
6. It has an extremely fast detection speed and can be detected at any time. The measurement of each sample is completed in a short period of time.
7. The sample does not need to be diluted, and the concentration range of the measurable sample is more than 100 times that of a conventional ultraviolet-visible photometer.
8. Easy-to-use data printing function, print reports directly through the built-in printer.
9. The service life of the xenon flash lamp can reach 1 billion times (up to 10 years). It can be used directly without preheating and can be tested at any time. No other consumables are required.
10. 100,000 pieces of data can be stored in each subdirectory.
11. The lowest detection limit in fluorometer mode can reach 0.5pg/μL (only for SP-MUV1000F).
Specifications
Model | SP-MUV1000 | SP-MUV1000F | |
Wavelength range | 190-850nm | 190-850nm | |
Sample capacity | 0. 5-2u I | 0. 5-2ul | |
Optical distance | 0. 05mmx 0. 2mm、 1.0mm (automatic switchover) | 0. 05mmx 0. 2mm、 1.0mm | |
(automatic switchover ) | |||
Light source/life | Xenon lamp/109 times | Xenon lamp/109 times | |
Detector | 2048 element linear CCD array | 2048 element linear CCD array | |
Spectral bandwidth | 2nm | 2nm | |
Absorbed light accuracy | 0. 003Abs | 0.003Abs | |
Absorbance accuracy | ±1% (7. 332Abs at 260nm) | ±1% (7. 332Abs at 260nm) | |
Absorbance range | (Equivalent to 10mm) 0.04-300A | (Equivalent to 10mm) 0.04-300A | |
Nucleic acid concentration range | 2ng/ μ L dsDNA~ | 2ng/ μ L dsDNA~ | |
15000ng/ μ L | 15000ng/ μ L | ||
dsDNA | dsDNA | ||
Detection time | ≤5 s | ≤5 s | |
OD600 | Absorbance range | 0~4. 000 Abs | 0~4. 000 Abs |
Absorbance stability | [0,3 )≤0. 5%, | [0,3 )≤0. 5%, | |
[3,4) ≤1% | [3,4) ≤1% | ||
Absorbance repeatability | [0,3 )≤0. 5%, | [0,3 )≤0. 5%, | |
[3,4) ≤1% | [3,4) ≤1% | ||
Absorbance accuracy | [0,3)< 0.005A+1%; [3 ,4) ≤2% | [0,3)< 0.005A+1%; [3 ,4) ≤2% | |
Fluorescence detection | / | Excitation wavelength: 460nm | |
Emission wavelength: 525nm | |||
Fluorescence detection | Linearity | / | R2>0. 995 |
Repeatability | / | <1.5% | |
Stability | / | <1.5% | |
Built-in thermal printer | Built-in thermal printer | ||
Data output mode | USB | USB | |
Material of sample base | Quartz optical fiber and high hard aluminum | Quartz optical fiber and high hard aluminum | |
Input voltage | VAC 100-240V ; VDC 24V 2A | VAC 100-240V; VDC 24V 2A | |
Power | <15W (Standby: 5W) | <15W (Standby: 5W) | |
Dimensions(W*D*H) | 208*290*180mm | 208*290*180mm | |
Weight | 3.2 kg | 3.2 kg |
Reagents and applications corresponding to different fluorescence channels
Channel | Excitation Wavelength | Common Fluorescent Reagents | Application |
Blue channel(Standard configuration) | 460±20nm | PicoGreen, oligreen, RiboGreenR, GFP, Protein, Fluorescein | dsDNA, ssDNA,
Green fluorescent protein GFP, Gene detection, Fluorescein detection, Protein quantification |
Uv channel(optional) | 365±20nm | Hoechst 33258, 4-MU, EnZCheK Caspase | Nucleic acid quantitation,
Plant GUS reporter gene detection, Apoptosis detection |
Green channel(optional) | 525±20nm | Rhodamine, Cy3, RFP Vybrant Cytotoxicity | Rhodamine assay,
Cy-3 fluorescent label detection, Red fluorescent protein RFP gene detection, Cell viability toxicity assay |
Red channel(optional) | 625±20nm | Cy5, Quant-iT RNA | Cy-5 fluorescent label detection,
RNA quantification |
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