As cell culture has gained more importance in research, it has evolved—and the incubator has evolved along with it
Carl earned his undergraduate degree in molecular and cellular biology from the University of Illinois and completed his master’s degree from Northwestern University’s Master of Biotechnology program. Prior to PHCNA, Carl was involved in various areas of biomedical research including cell and molecular biology, microbiology, cancer research, and clinical diagnostic assay development. Prior affiliations have included Northwestern’s School of Medicine and Nanosphere (now Luminex), a nano-biotech company in Illinois.
Q: HOW HAS CELL CULTURE EVOLVED OVER THE LAST FEW DECADES?
A: Cell culture has been around for several decades now, in one form or another. Historically cell culture was used in research models and R&D in the pharma world. It’s been evolving for many years now, especially in the last five or so years, with things like scaffoldings and 3D cell cultures where we can really start to mimic tissues much more robustly. As cell culture has gained more importance in study and primary cell lines—cells taken directly from a biopsy or from a patient and placed into culture—which are delicate in nature, it has had to evolve. And the incubator has evolved along with it.
Q: WHAT SORT OF ENVIRONMENT IS NEEDED TO MAINTAIN CELL CULTURE IN CLINICAL LABS?
A: Cells that are very hearty need your standard five percent CO2 and temperature and humidity levels. With the clinical method, on the other hand, you really need the tightest control of temperature and gases. So power, versatility, and precision-controlled environments—CO2 levels, oxygen levels, and temperature levels—become that much more important.
Our central principle of incubator design is to have the best cell health in mind. I use the analogy of building in the best brains and nerves, meaning having the smartest microprocessor brain controlling everything and the highest-quality sensors for things like temperature, gas, and other diagnostics. If you open a door in the incubator, you break that perfect environment for the cell because gases rush out and temperature might change. What we want to have is the most reactive system that recognizes that deviation in gas or temperature, and that tells those parameters in real time to get back to their normal environment. It has to recognize that the CO2 level is off and pull it back very quickly without overshooting it. It has to instantly recognize that the temperature is off and recover in the most uniform way. That’s the concept we’ve built in as our incubator has evolved into its latest form—real-time monitoring and control and recovery of incubator parameters, so we can properly mimic the human or animal body from which these cells came.
Q: WHAT SORTS OF CLINICAL APPLICATIONS REQUIRE THE TIGHTEST PRECISION-CONTROLLED CELL ENVIRONMENTS?
A: One example is embryo implantation for in vitro fertilization. Another is CAR-T cell therapy for targeting circulating and solid tumors, where essentially, we harvest the blood and the immune cells from a cancer patient, study them, manipulate them, and reinject them into the patient in order to fight off the cancer. The incubator is a central part of that process. There’s a long incubation period where we have to let these cells grow and they’re outside of their natural environment, so we have to make sure they’re happy with the parameters. Once you cross from research into the more clinical realm, the importance of cell health along its entire journey, from extraction from a patient, into a cell culture dish and an incubator, and then back into a patient, is extremely heightened.
Q: ASIDE FROM MAINTAINING THE RIGHT PARAMETERS FOR CELL CULTURE, WHAT OTHER FUNCTIONS DOES THE INCUBATOR SERVE?
A: In addition to controlling parameters in cells as they’re growing, there’s also this idea of protecting cells from microbial contamination and cross contamination. Microbes are all around us. They are adapted to living in the world, on our skin, and in our guts. We co-exist with these natural enemies of the cell culture, so we need an incubator that is going to keep them at bay.