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Liquid Chromatograph, HPLC-2025

  • Pump Flow Rate Range: 0.001~10.000mL/min
  • Autosampler Injection Range:0-100μL
  • High sensitivity detector
  • Powerful data processing software

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Description

The HPLC-2025 Liquid Chromatograph is a product with higher quality, more reliability and accuracy.

Features

  • Reliability

With the reliable design and high-quality components, HPLC-2025 can operate in good condition for a long time

  • Precision

The precision and accuracy of the results are guaranteed with the pumping and sampling technology, high sensitivity detector, and powerful data processing software.

  • Ease of use

The operation is more convenient and efficient with a variety of user-friendly designs.

Specifications

HPLC-2025P1/P2/P4 Pump

Features

Precision

  • Excellent repeatability

The precise gradient change and retention time are guaranteed with the reciprocating tandem plunger pump and pressure dynamic suppression algorithm. The repeatability of retention time is less than 0.2%.

Reliability

  • The reliable design makes service life longer
  • Material

The cam is integrally processed from high-hardness alloy steel and combined with high-frequency heat treatment technology, which makes the surface of the cam is more wear-resistant (the hardness is above 55HRC), and the service life is longer.

  • Power

The high-power customized motor, NSK bearings, and independent air duct make the power and life even better.

  • Gearing

Self-lubricating and wear-resistant materials are used for the piston drive mechanism. Also, the inner wall rifling design prevents the piston from accidentally locking, making the gearing more reliable.

  • Plunger

The suspended floating plunger can adapt to the working conditions automatically, which is convenient for disassembly and prevents the eccentric wear of the sealing ring effectively. With the special sealing structure and the automatic cleaning of the plunger, the pump seal is more reliable.

  • Ease of use
  • Equipped with an online automatic rinsing mechanism.

When the pump is working, the rinsing pump is automatically turned on and runs periodically, which can effectively prevent the crystallization of buffer salts and the growth of microorganisms, and prolong the service life of the plunger and plunger sealing.

  • Easy to purge

Both the workstation and the instrument panel are equipped with a purging function, making it easier to purge.

  • Intelligent solvent switching to improve efficiency

Equipped with a 4-channel solvent selection valve for binary pump. The solvent can be automatically switched according to the method.

  • Independent 4-channel gradient proportioning valve, easy for maintenance

The gradient proportioning valve of the quaternary pump adopts a 4-channel independent design. The intelligent monitoring and diagnosis can realize real-time monitoring and independent replacement of each channel, the whole design can reduce maintenance costs effectively.

Specification

Flow Rate Range 0.001mL/min~10.000mL/min
Pump Type Isocratic(P1), Binary(P2), Quaternary(P4)
Maximum Pressure 62MPa
Plunger Rinsing Supported

 

HPLC-2025AS Autosampler

Features

  • Precision
  • Precise injection volume

The autosampler adopts an integrated constant pressure needle and a precise syringe pump, which ensures accurate injection volume and excellent linearity, making the results more precise and accurate.

  • Low carryover to make results more accurate

The sampling needle is highly polished for the outer surface and passivated for the inner surface, which can effectively reduce the sample residue. The carryover is much lower when needle washing of the outer and inner surfaces are performed.

  • Reliability
  • Design of needle

With our integrated constant pressure sampling needle, the air pressure inside and outside the sample bottle can be balanced during sampling; the injection port is set on the sidewall to prevent bottle pad debris from clogging the needle during puncture.

  • Design of degassing unit of washing liquid

The built-in autosampler degassing unit can be used for degassing of washing liquid, avoiding interference caused by air bubbles.

  • Design of self-compensation injection pump

The maintenance-free self-lubricating guide device and self-compensating sliding screw are used for real-time compensation of the slight wear, and it has excellent motion accuracy and is more durable.

  • Ease of use
  • Preloaded sample injection mode, the injection time can be shortened to 5s, which greatly improves the efficiency.
  • A variety of injection modes combined with sample temperature control technology to satisfy different detection needs such as rare and unstable samples.
  • The dehumidification function is optionally equipped to avoid the generation of condensed water, effectively prevent the sample from being diluted, and ensure accurate results.
  • The maintenance is more convenient and efficient with the in-front injection valve.

Specification

Sample Capacity 108, 2mL(96&384 well plates)
Injection Range 0-100μL
Injection Mode Low-loss, microliter carrying, full-loop
Injection Time Minimum to 5 s
Carryover 0.003%
Degassing Unit Online degassing(optional)
Temperature Control 4-40 ℃(optional)

HPLC-2025CO Column Oven

Features

  • Precision

Precise temperature control ensures good separation and repeatability.

Using fuzzy PID intelligent temperature control algorithm, ban-based air circulation, and multiple insulation layer design, the column temperature is more accurate, stable, and uniform, the temperature stability is ±0.1 ℃, and the temperature for each analysis are constant and consistent.

  • Reliability

Triple protection, more safe to use.

Real-time liquid leak protection, intelligent monitoring of Peltier and cavity temperature, over-temperature power-off protection, triple safety protection designs, effectively prevents accidental liquid leakage and dangers caused by over-heating.

Specification

Operating Principle Peltier element and fan-based forced air
Temperature Range 5 ℃ above ambient to 85 ℃

10 ℃ below ambient to 85 ℃

 

HPLC-2025UVD UV-Vis Detector

Features

  1. Precision
  • Low LOD

The high-throughput optical path, reference subtraction algorithm, and ultra-precise signal acquisition circuit ensure the detector’s excellent sensitivity.

  • Wide linearity

The UVD covers a wide linear range of 2.5AU, making it easier to analyze high concentration samples.

  1. Reliability

The transmission type deuterium lamp and static light path allow the light source switching without moving of lamp, which is more reliable.

With the design of the optical unit and the light source module, the service life is much longer.

  1. Ease of use
  • Full-band spectral scanning

A full-band spectral scanning can be performed to easily find the optimal absorption wavelength of the target analyte.

  • Wavelength time programming

Wavelength can be set for different analytes during different periods, to accomplish the high-sensitivity analysis of complex samples.

  • Good maintenance and high safety
  • Built-in mercury lamp for wavelength calibration.
  • Over-temperature protection of light source.
  • Automatic tracking of light energy.
  • With in-front light source and flow celland Liquid leak sensor.

Specification

Light Source Deuterium(transmission type) and Tungsten lamp
Wavelength Range 190-800nm
Wavelength Accuracy +/- 1nm
Wavelength Precision +/- 0.1nm
Linearity >2.5 AU
Wavelength Calibration Mercury lamp

HPLC-2025DAD Diode Array Detector

Features

  • Precision
  • Spectral similarity alignment and peak purity determination are performed for precise qualitative and quantitative analysis.

DAD provides full-spectrum information. The compounds can be identified by similarity comparison in the spectral database. Peak purity determination can be performed in the workstation.

  • High sensitivity—Close to UV Detector

High sensitivity mode can be performed for low concentration samples, the signal-to-noise ratio of DAD can be reached to the same level of UVD.

  • Achieving more accurate inspection data by high-resolution spectra.

When compound detection is performed in high-resolution mode, a finer spectral map can be output for better analysis of complex samples.

  • Ease of use
  1. Supports 12-channel real-time data collection
  2. Significantly enhance the analysis efficiency by:
  • Up to 12 wavelengths can be set for real-time detection;
  • Real-time analysis at a high sampling rate up to 150Hz;
  • Full-spectrum detection.

Specification

Light Source Deuterium (transmission type) & Tungsten lamp
Wavelength Range 190~800nm
Slit Width 1nm, 2nm, 4nm, 8nm
Wavelength Accuracy +/- 1nm
Wavelength Precision +/- 0.1nm
Linearity > 2.0 AU
Wavelength Calibration Holmium glass

HPLC-2025FLD Fluorescence Detector

Specification

Light Source 150W Xe lamp
Wavelength Range(Excitation) 200 to 850nm Zero order
Wavelength Range(Emission) 250 to 900nm Zero order
Detection Sensitivity (Raman peak of water S/N) S/N ≥ 900 (Baseline method)
Wavelength Accuracy ±3nm
Wavelength Repeatability ±0.5nm

HPLC-2025ELSD Evaporative Light-Scattering Detector

Specification

Noise ≤0.05mV(1.0mL/min 100% Methanol, Gain 1.00)
Drift ≤0.3mV/30 min(1.0mL/min 100% Methanol, Gain 1.00)
Min. Detection Limit ≤5.0×10-6 g/mL(500pg)

HPLC-2025WS Workstation

Features

  • User-friendly
  • Ease of use: The workstation interface is flat designed to quickly complete method development, data acquisition, data processing, report editing and printing, etc..
  • Precision
  • Powerfuldata-processing capability: Up to 25 integration events and 3 quantitative calculation methods (external standard method, internal standard method, and normalization method) for complex data.
  • Compliance
  • Database storage: With the professional database system, the encryption of original files and security of raw data can be guaranteed.
  • Backup and restore: Backup and restore of data can be easily performed in the workstation, which is convenient for users.
  • Multi-level user system: Supports customized multi-level system for access to data, ensuring the effectiveness of the system.

 

 

 

 

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    Contact Info

    Rm. 2014, Bldg. 3, Ligaoguojihuayuan, No. 1222, West Aoti Road, Lixia District, Jinan, Shandong

    Phone: +86-531-88982330

    Fax: +86-531-88983691

    Web: www.bioevopeak.com

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